Review



alexa fluor 488 conjugated mouse anti human cd61  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Bio-Rad alexa fluor 488 conjugated mouse anti human cd61
    In Vitro Assessment of Platelet Reactivity by Aggregometry and MF (A) Percent maximal aggregation of platelets from patients on dual antiplatelet therapy (DAPT) (n = 27) in response to adenosine diphosphate (ADP) (20 μmol/L) and TRAP-6 (10 μmol/L). Results are compared with healthy donors (n = 15). (B) Mean fluorescence intensity (FI) (n = 8 clotting events per individual) of accumulated platelets from whole blood of healthy donors vs patients on DAPT using a PDMS (polydimethylsiloxane) device attached to a type I equine collagen–coated glass slide. Digital imagines were captured using an automated fluorescent microscope system ( t = 900 seconds). (C) Photomicrograph of a fabricated collagen-coated chip and deposition of platelets labeled with Alexa Fluor 488–conjugated mouse anti-human <t>CD61</t> from healthy donors or DAPT-treated patients ( t = 900 seconds). (D) Mean platelet FI (n = 8 clotting events per individual) of accumulated platelets from WB of the same individuals from using collagen-coated chips and an integrated microfluidic (MF) device. (E) Maximal thrombus area (μm 2 ) occupied by calcein-labeled platelets from healthy donors vs patients (n = 10 mice per group, 10 individuals for each of the 2 groups, 3 arteriole injuries per mouse). The central box represents the values between the 10th and 90th percentiles, and the middle line is the mean. Statistical significance was determined using Mann-Whitney U test. ∗∗∗ P < 0.001 relative to healthy donors; ∗∗∗ P < 0.001 ADP relative to TRAP-6 for patients on DAPT.
    Alexa Fluor 488 Conjugated Mouse Anti Human Cd61, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd61+alexa+488/Mouse+anti+Human+CD61/pmc12390928-45-5-20
    Average 93 stars, based on 36 article reviews
    alexa fluor 488 conjugated mouse anti human cd61 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Preclinical Efficacy of a Hemostatic Agent in Overcoming Dual Antiplatelet Therapy"

    Article Title: Preclinical Efficacy of a Hemostatic Agent in Overcoming Dual Antiplatelet Therapy

    Journal: JACC: Basic to Translational Science

    doi: 10.1016/j.jacbts.2025.101356

    In Vitro Assessment of Platelet Reactivity by Aggregometry and MF (A) Percent maximal aggregation of platelets from patients on dual antiplatelet therapy (DAPT) (n = 27) in response to adenosine diphosphate (ADP) (20 μmol/L) and TRAP-6 (10 μmol/L). Results are compared with healthy donors (n = 15). (B) Mean fluorescence intensity (FI) (n = 8 clotting events per individual) of accumulated platelets from whole blood of healthy donors vs patients on DAPT using a PDMS (polydimethylsiloxane) device attached to a type I equine collagen–coated glass slide. Digital imagines were captured using an automated fluorescent microscope system ( t = 900 seconds). (C) Photomicrograph of a fabricated collagen-coated chip and deposition of platelets labeled with Alexa Fluor 488–conjugated mouse anti-human CD61 from healthy donors or DAPT-treated patients ( t = 900 seconds). (D) Mean platelet FI (n = 8 clotting events per individual) of accumulated platelets from WB of the same individuals from using collagen-coated chips and an integrated microfluidic (MF) device. (E) Maximal thrombus area (μm 2 ) occupied by calcein-labeled platelets from healthy donors vs patients (n = 10 mice per group, 10 individuals for each of the 2 groups, 3 arteriole injuries per mouse). The central box represents the values between the 10th and 90th percentiles, and the middle line is the mean. Statistical significance was determined using Mann-Whitney U test. ∗∗∗ P < 0.001 relative to healthy donors; ∗∗∗ P < 0.001 ADP relative to TRAP-6 for patients on DAPT.
    Figure Legend Snippet: In Vitro Assessment of Platelet Reactivity by Aggregometry and MF (A) Percent maximal aggregation of platelets from patients on dual antiplatelet therapy (DAPT) (n = 27) in response to adenosine diphosphate (ADP) (20 μmol/L) and TRAP-6 (10 μmol/L). Results are compared with healthy donors (n = 15). (B) Mean fluorescence intensity (FI) (n = 8 clotting events per individual) of accumulated platelets from whole blood of healthy donors vs patients on DAPT using a PDMS (polydimethylsiloxane) device attached to a type I equine collagen–coated glass slide. Digital imagines were captured using an automated fluorescent microscope system ( t = 900 seconds). (C) Photomicrograph of a fabricated collagen-coated chip and deposition of platelets labeled with Alexa Fluor 488–conjugated mouse anti-human CD61 from healthy donors or DAPT-treated patients ( t = 900 seconds). (D) Mean platelet FI (n = 8 clotting events per individual) of accumulated platelets from WB of the same individuals from using collagen-coated chips and an integrated microfluidic (MF) device. (E) Maximal thrombus area (μm 2 ) occupied by calcein-labeled platelets from healthy donors vs patients (n = 10 mice per group, 10 individuals for each of the 2 groups, 3 arteriole injuries per mouse). The central box represents the values between the 10th and 90th percentiles, and the middle line is the mean. Statistical significance was determined using Mann-Whitney U test. ∗∗∗ P < 0.001 relative to healthy donors; ∗∗∗ P < 0.001 ADP relative to TRAP-6 for patients on DAPT.

    Techniques Used: In Vitro, Fluorescence, Coagulation, Microscopy, Labeling, MANN-WHITNEY

    Related Articles

    Flow Cytometry:

    Article Title: Bioreactor for isolation of rare cells and methods of use
    Article Snippet: .. Primary antibodies used for flow cytometry and cell sorting include mouse anti-human CD41-FITC or -APC, mouse anti-human CD42b-PE, rat anti-mouse CD41, mouse anti-human CD38-PE-Cy7 (eBioscience), mouse anti-human CD61-Alexa 488 (AbD Serotec), anti-Annexin V-PE (BD Biosciences), mouse antihuman CD34-PE (Invitrogen), and mouse anti-human SIRPa-PE (Santa Cruz). .. Phalloidin-TRITC was purchased from Invitrogen.

    FACS:

    Article Title: Bioreactor for isolation of rare cells and methods of use
    Article Snippet: .. Primary antibodies used for flow cytometry and cell sorting include mouse anti-human CD41-FITC or -APC, mouse anti-human CD42b-PE, rat anti-mouse CD41, mouse anti-human CD38-PE-Cy7 (eBioscience), mouse anti-human CD61-Alexa 488 (AbD Serotec), anti-Annexin V-PE (BD Biosciences), mouse antihuman CD34-PE (Invitrogen), and mouse anti-human SIRPa-PE (Santa Cruz). .. Phalloidin-TRITC was purchased from Invitrogen.



    Similar Products

    alexa fluor 488 conjugated mouse anti human cd61  (Bio-Rad)
    93
    Bio-Rad alexa fluor 488 conjugated mouse anti human cd61
    In Vitro Assessment of Platelet Reactivity by Aggregometry and MF (A) Percent maximal aggregation of platelets from patients on dual antiplatelet therapy (DAPT) (n = 27) in response to adenosine diphosphate (ADP) (20 μmol/L) and TRAP-6 (10 μmol/L). Results are compared with healthy donors (n = 15). (B) Mean fluorescence intensity (FI) (n = 8 clotting events per individual) of accumulated platelets from whole blood of healthy donors vs patients on DAPT using a PDMS (polydimethylsiloxane) device attached to a type I equine collagen–coated glass slide. Digital imagines were captured using an automated fluorescent microscope system ( t = 900 seconds). (C) Photomicrograph of a fabricated collagen-coated chip and deposition of platelets labeled with Alexa Fluor 488–conjugated mouse anti-human <t>CD61</t> from healthy donors or DAPT-treated patients ( t = 900 seconds). (D) Mean platelet FI (n = 8 clotting events per individual) of accumulated platelets from WB of the same individuals from using collagen-coated chips and an integrated microfluidic (MF) device. (E) Maximal thrombus area (μm 2 ) occupied by calcein-labeled platelets from healthy donors vs patients (n = 10 mice per group, 10 individuals for each of the 2 groups, 3 arteriole injuries per mouse). The central box represents the values between the 10th and 90th percentiles, and the middle line is the mean. Statistical significance was determined using Mann-Whitney U test. ∗∗∗ P < 0.001 relative to healthy donors; ∗∗∗ P < 0.001 ADP relative to TRAP-6 for patients on DAPT.
    Alexa Fluor 488 Conjugated Mouse Anti Human Cd61, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd61+alexa+488/Mouse+anti+Human+CD61/pmc12390928-45-5-20
    Average 93 stars, based on 1 article reviews
    alexa fluor 488 conjugated mouse anti human cd61 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    cd61 alexa fluor 488  (Bio-Rad)
    93
    Bio-Rad cd61 alexa fluor 488
    ACCEPT gates used for the automated enumeration of leukocytes, leukocyte-derived Extracellular Vesicles (ldEVs), platelets, and red blood cells in blood A with EpCAM enrichment and B without EpCAM enrichment.
    Cd61 Alexa Fluor 488, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd61+alexa+488/Mouse+anti+Human+CD61/pmc06721753-74-40-46
    Average 93 stars, based on 1 article reviews
    cd61 alexa fluor 488 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    mouse anti human cd61 alexa 488  (Bio-Rad)
    93
    Bio-Rad mouse anti human cd61 alexa 488
    ACCEPT gates used for the automated enumeration of leukocytes, leukocyte-derived Extracellular Vesicles (ldEVs), platelets, and red blood cells in blood A with EpCAM enrichment and B without EpCAM enrichment.
    Mouse Anti Human Cd61 Alexa 488, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd61+alexa+488/Mouse+anti+Human+CD61/us09920295-462-25-29
    Average 93 stars, based on 1 article reviews
    mouse anti human cd61 alexa 488 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    mouse anti human cd61 alexa fluor 488  (Bio-Rad)
    93
    Bio-Rad mouse anti human cd61 alexa fluor 488
    ACCEPT gates used for the automated enumeration of leukocytes, leukocyte-derived Extracellular Vesicles (ldEVs), platelets, and red blood cells in blood A with EpCAM enrichment and B without EpCAM enrichment.
    Mouse Anti Human Cd61 Alexa Fluor 488, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd61+alexa+488/Mouse+anti+Human+CD61/pm21126256-72-47-53
    Average 93 stars, based on 1 article reviews
    mouse anti human cd61 alexa fluor 488 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    In Vitro Assessment of Platelet Reactivity by Aggregometry and MF (A) Percent maximal aggregation of platelets from patients on dual antiplatelet therapy (DAPT) (n = 27) in response to adenosine diphosphate (ADP) (20 μmol/L) and TRAP-6 (10 μmol/L). Results are compared with healthy donors (n = 15). (B) Mean fluorescence intensity (FI) (n = 8 clotting events per individual) of accumulated platelets from whole blood of healthy donors vs patients on DAPT using a PDMS (polydimethylsiloxane) device attached to a type I equine collagen–coated glass slide. Digital imagines were captured using an automated fluorescent microscope system ( t = 900 seconds). (C) Photomicrograph of a fabricated collagen-coated chip and deposition of platelets labeled with Alexa Fluor 488–conjugated mouse anti-human CD61 from healthy donors or DAPT-treated patients ( t = 900 seconds). (D) Mean platelet FI (n = 8 clotting events per individual) of accumulated platelets from WB of the same individuals from using collagen-coated chips and an integrated microfluidic (MF) device. (E) Maximal thrombus area (μm 2 ) occupied by calcein-labeled platelets from healthy donors vs patients (n = 10 mice per group, 10 individuals for each of the 2 groups, 3 arteriole injuries per mouse). The central box represents the values between the 10th and 90th percentiles, and the middle line is the mean. Statistical significance was determined using Mann-Whitney U test. ∗∗∗ P < 0.001 relative to healthy donors; ∗∗∗ P < 0.001 ADP relative to TRAP-6 for patients on DAPT.

    Journal: JACC: Basic to Translational Science

    Article Title: Preclinical Efficacy of a Hemostatic Agent in Overcoming Dual Antiplatelet Therapy

    doi: 10.1016/j.jacbts.2025.101356

    Figure Lengend Snippet: In Vitro Assessment of Platelet Reactivity by Aggregometry and MF (A) Percent maximal aggregation of platelets from patients on dual antiplatelet therapy (DAPT) (n = 27) in response to adenosine diphosphate (ADP) (20 μmol/L) and TRAP-6 (10 μmol/L). Results are compared with healthy donors (n = 15). (B) Mean fluorescence intensity (FI) (n = 8 clotting events per individual) of accumulated platelets from whole blood of healthy donors vs patients on DAPT using a PDMS (polydimethylsiloxane) device attached to a type I equine collagen–coated glass slide. Digital imagines were captured using an automated fluorescent microscope system ( t = 900 seconds). (C) Photomicrograph of a fabricated collagen-coated chip and deposition of platelets labeled with Alexa Fluor 488–conjugated mouse anti-human CD61 from healthy donors or DAPT-treated patients ( t = 900 seconds). (D) Mean platelet FI (n = 8 clotting events per individual) of accumulated platelets from WB of the same individuals from using collagen-coated chips and an integrated microfluidic (MF) device. (E) Maximal thrombus area (μm 2 ) occupied by calcein-labeled platelets from healthy donors vs patients (n = 10 mice per group, 10 individuals for each of the 2 groups, 3 arteriole injuries per mouse). The central box represents the values between the 10th and 90th percentiles, and the middle line is the mean. Statistical significance was determined using Mann-Whitney U test. ∗∗∗ P < 0.001 relative to healthy donors; ∗∗∗ P < 0.001 ADP relative to TRAP-6 for patients on DAPT.

    Article Snippet: The thrombin inhibitor H-D-Phe-Pro-Arg-chloromethylketone (PPACK), Alexa Fluor 488–conjugated mouse anti-human CD61, and Alexa Fluor 647–conjugated human fibrinogen were obtained from Bio-Rad and Thermo Fisher Scientific, respectively.

    Techniques: In Vitro, Fluorescence, Coagulation, Microscopy, Labeling, MANN-WHITNEY

    ACCEPT gates used for the automated enumeration of leukocytes, leukocyte-derived Extracellular Vesicles (ldEVs), platelets, and red blood cells in blood A with EpCAM enrichment and B without EpCAM enrichment.

    Journal: Cells

    Article Title: Leukocyte-Derived Extracellular Vesicles in Blood with and without EpCAM Enrichment

    doi: 10.3390/cells8080937

    Figure Lengend Snippet: ACCEPT gates used for the automated enumeration of leukocytes, leukocyte-derived Extracellular Vesicles (ldEVs), platelets, and red blood cells in blood A with EpCAM enrichment and B without EpCAM enrichment.

    Article Snippet: Blood was incubated with the nuclear dye Hoechst 33342 (Invitrogen, cat. # H3570), the fluorescently tagged monoclonal antibodies CD45-PerCP (clone HI30 Invitrogen, cat. # MHCD4531) recognizing leukocytes, CD235a-Alexa Fluor ® 647 (clone YTH89.1, bio-rad, MCA 506A647) antibodies recognizing erythrocytes and CD61-Alexa Fluor ® 488 (clone Y2.51, bio-rad, cat. # MCA 2588A488) antibodies recognizing platelets.

    Techniques: Standard Deviation

    Examples of objects detected in the immunofluorescence image data sets of blood samples without EpCAM enrichment. The objects fall in the ACCEPT gates, the names of which are indicated vertically. Panel ( A ) shows examples of ACCEPT thumbnails. Scale bars indicate 6.4 μm. Panel ( B ) shows scatter plots of the mean intensity of the Hoechst versus the mean intensity of CD45-PerCP and the mean intensity of CD61-Alexa 488 versus the mean intensity of CD235a-Alexa 647. Blue dots represent single events falling in the respective gate.

    Journal: Cells

    Article Title: Leukocyte-Derived Extracellular Vesicles in Blood with and without EpCAM Enrichment

    doi: 10.3390/cells8080937

    Figure Lengend Snippet: Examples of objects detected in the immunofluorescence image data sets of blood samples without EpCAM enrichment. The objects fall in the ACCEPT gates, the names of which are indicated vertically. Panel ( A ) shows examples of ACCEPT thumbnails. Scale bars indicate 6.4 μm. Panel ( B ) shows scatter plots of the mean intensity of the Hoechst versus the mean intensity of CD45-PerCP and the mean intensity of CD61-Alexa 488 versus the mean intensity of CD235a-Alexa 647. Blue dots represent single events falling in the respective gate.

    Article Snippet: Blood was incubated with the nuclear dye Hoechst 33342 (Invitrogen, cat. # H3570), the fluorescently tagged monoclonal antibodies CD45-PerCP (clone HI30 Invitrogen, cat. # MHCD4531) recognizing leukocytes, CD235a-Alexa Fluor ® 647 (clone YTH89.1, bio-rad, MCA 506A647) antibodies recognizing erythrocytes and CD61-Alexa Fluor ® 488 (clone Y2.51, bio-rad, cat. # MCA 2588A488) antibodies recognizing platelets.

    Techniques: Immunofluorescence